Oligonucleotide Purification Methods
When selecting a purification method for oligonucleotide synthesis, it is essential to consider factors such as oligo length, modifications, as well as intended application and yield. We provide a range of customizable purification options to enhance quality and remove synthesis by-products. The higher the targeted quality of the oligonucleotides, the less yield is produced for a given scale and length.
Purification Methods
We offer a range of purification options and allow customers to request combinations to optimize product quality and effectively eliminate synthesis by-products. For optimal reproducibility, we recommend RP-HPLC purification for oligonucleotides 25 bases or longer, as well as for all modified oligonucleotides and those intended for diagnostic applications.
Which oligonucleotide purification method is right for my oligo?
The right purification method depends on the length, modifications and intended application of your oligonucleotide. The overview below summarises our recommendations:
| Method | Valid for | Good to know |
|---|---|---|
| Desalting | Standard PCR primers up to ~20 bases | Removes truncates <6 nt and chemical residues; not recommended for qPCR or other highly sensitive applications |
| HPLC (RP-HPLC, IEX-HPLC) | All modified and dye-labeled oligos, diagnostic oligos | Standard for all modified and RNA oligos – free of charge for modified oligonucleotides |
| Duplex HPLC | siRNA duplexes and other double-stranded oligos | Second HPLC of the hybridized strands removes excess single strands |
Service on request
- PAGE – for very long oligos (50 nt or longer) and applications requiring the highest purity. Single-base resolution and >95% full-length product, with approx. 50% lower yield and a longer delivery time.
- Cartridge – for NGS primers and applications where cross-contamination must be avoided. Single-use cartridges; around 70% purity for unlabeled oligos.
Not sure which method fits your application? Our team will be happy to recommend the optimal purification for your oligo design – just contact us.
For detailed information please read Purification Methods in the Resource section.
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