Purification Methods
When selecting a purification method for oligonucleotide synthesis, it is essential to consider factors such as oligo length, modifications, as well as intended application and yield. We provide a range of customizable purification options to enhance quality and remove synthesis by-products. The higher the targeted quality of the oligonucleotides, the less yield is produced for a given scale and length.
Purification Methods
We offer a range of purification options and allow customers to request combinations to optimize product quality and effectively eliminate synthesis by-products. For optimal reproducibility, we recommend RP-HPLC purification for oligonucleotides 25 bases or longer, as well as for all modified oligonucleotides and those intended for diagnostic applications.
Method
Description
Desalting
Removal of truncates <6 nt and chemical residues
RP-HPLC
Removal of deletions and contaminants utilizing oligo hydrophobicity
IEX HPLC
Removal of deletions and contaminants utilizing oligo charge
Duplex HPLC
2nd HPLC of the hybridized oligos for removal of excess single strands
Cartridge
Removal of truncates and chemical residues utilizing 5’DMT interaction
PAGE
Removal of deletions and contaminants utilizing molecular weight
For detailed information please read Purification Methods in the Resource section.
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