START Breakthroughs, One Oligo At A Time
Special Oligonucleotide Synthesis
We specialize in advanced oligonucleotide synthesis, producing both standard and complex oligonucleotides for a wide range of applications.
Our services include TRImer oligos for library construction, antisense oligos, and custom primers / probes designed specifically for research and diagnostic use.
Find out how our expertise can help you get the research you need, fast!
TRImer Oligos
The use of 2′-deoxynucleoside TRImer phosphoramidites provides an efficient and effective approach to oligonucleotide-directed mutagenesis.
By eliminating codon bias and avoiding stop codons, TRImer-oligonucleotide libraries allow for greater control over codon abundance, making them a valuable tool for exploring functional protein regions.
Antisense Oligos
We provide high-quality therapeutic oligonucleotides, including ASOs, siRNA, and Gapmers, for gene silencing and therapeutic applications. With extensive expertise in oligonucleotide modifications—such as backbone alterations, base substitutions, and fluorescent dye labeling—we deliver customized solutions for diverse research and diagnostic needs, offering flexible purification options and packaging formats.
Diagnostics
Our range of real-time qPCR probes and primers includes customizable quenchers and dyes designed for a variety of applications such as SNP detection, and expression profiling.
We offer flexible synthesis options, including 5′ Quencher-3′ Reporter, internal dyes and quenchers, as well as Inosin/Wobbles, along with tailored solutions for diagnostic applications.
Custom Oligonucleotide Synthesis
We provide a comprehensive range of chemical modifications for both oligonucleotide backbones and additional customizations.
You can select from a variety of purification methods and supplementary quality control options to guarantee that your oligonucleotides are precisely tailored to your specific application requirements.
Length
We offer oligonucleotide synthesis ranging from 2 to 150 nucleotides. The synthesis of very short oligonucleotides may present challenges in precipitation, while those exceeding 140 nucleotides may exceed mass spectrometry limits for quality control.
For longer oligonucleotides, please inquire about customised options.
Scales and Yields
We offer oligo synthesis at common scales, suitable for various applications. Our team ensures quality and optimal yield, adjusting for specific modifications and purifications as needed to support your research and diagnostic requirements.
Backbone
The chemical modification of the backbone in oligonucleotide synthesis is a process that involves mainly altering the phosphate backbone in order to enhance stability, nuclease resistance, binding affinity, and often melting point (Tm) to improve performance in various applications.
Modifications
Chemical modifications to oligonucleotides, such as the use of amino modifiers, spacers, etc., can enhance oligo stability, delivery, and target specificity. Using fluorescent tags for oligonucleotide labeling is a powerful technique with numerous applications in diagnostics and research.
Purification
When selecting a purification method for oligonucleotide synthesis, factors such as oligo length, modifications, required yield, and intended application are crucial. We offer various purification options and allow customers to request combinations to optimize product quality and effectively remove by-products from synthesis processes.
Delivery Format and Packaging
Our oligo products are available in single tubes, multiwell plates, or bulk, either lyophilized or dissolved in H₂O or buffers at your desired concentration. We also offer custom vial labeling, solutions at specified concentrations, and flexible aliquoting options to fit your needs.
Quality and Quality Control
We guarantee the highest quality in our synthesized DNA and RNA oligos through rigorous quality control testing, including RP-HPLC, IEX-HPLC, MALDI-MS, ESI-MS, and PAGE. All oligos are produced under ISO 13485 standards to ensure exceptional purity and reliability.
Additional Services
We offer endotoxin-free production and endotoxin-level (LAL) testing to guarantee that our oligonucleotides meet the required safety standards for sensitive applications. Our Duplex Setup service provides pre-annealed oligo pairs, and we provide comprehensive Certificate of Analysis (CoA) documentation for quality assurance purposes.
Production Time and Delivery
Custom oligo production times vary based on modification complexity, yield, and order size. More complex modifications and higher yields may require additional time. Shipment options include FedEx for both national (in Germany) and international deliveries, with the choice of dry ice or blue ice (gel packs) for temperature-sensitive shipments.