Fluorophores and Quenchers

We are experienced in fluorescence and quencher modifications for oligonucleotide synthesis. As a result, we are able to offer our customers a wide range of high quality dyes and quenchers that enable highly accurate detection results.

The dyes that we use include ATTO, Dyomic, ELITech, LiCor and many more.

In addition to these dyes, we also provide high-performance probes that are suitable for a range of molecular applications, such as qPCR and SNP detection. By partnering with us, you can be assured that your research projects will deliver reliable results.

List of Fluorophores

Fluorophores

Dye	λ (absorption, max)	λ (emission, max)	εmax [M-1cm-1]*	Quantum Yield*	CF260	Brightness*
FAM™	495	520	83.000	93%	0.22	77190
TET™	521	536	100.000	47%	0.17	47000
Yakima Yellow®	530	549	83.800	N/A	0.28	N/A
JOE™	529	555	75.000	61%	0.36	45750
HEX™	535	556	87.770	57%	0.30	50029
Cy3®	555	570	150.000	15%	0.03	22500
Cy3®(Sulfo)	548	563	162.000	15%	0.03	24300
Cy3B®	560	571	121.000	68%	0.04	82280
TAMRA™	557	583	84.000	10%	0.32	8400
Cy3.5®	581	596	116.000	35%	N/A	40600
Cy3.5®(Sulfo)	581	596	116.000	35%	N/A	40600
ROX™	578	604	82.000	94%	0.62	77080
Texas Red®	586	603	116.000	93%	0.23	107880
Cy5®	646	669	250.000	28%	0.13	70000
Cy5®(Sulfo)	646	669	271.000	20%	0.13	54200
Cy5.5®	683	694	209.000	20%	0.08	41800
Cy5.5®(Sulfo)	683	694	209.000	20%	0.08	41800
Cy7®	750	773	199.000	30%	0.04	59700
Cy7®(Sulfo)	750	773	240.000	30%	0.04	72000
IRDye®700	685	705	270.000	N/A	0.11	N/A
ATTO™ Cyan 500	450	495	45.000	80%	0.35	36000
ATTO™ 390	390	476	24.000	90%	0.46	21600
ATTO™ 425	439	485	45.000	90%	0.19	40500
ATTO™ 430LS	436	545	32.000	65%	0.32	20800
ATTO™ 465	453	506	75.000	75%	1.09	56250
ATTO™ 488	500	520	90.000	80%	0.22	72000
ATTO™ 495	498	526	80.000	20%	0.45	16000
ATTO™ 490LS	498	658	40.000	30%	0.39	12000
ATTO™ Rho110	507	531	100.000	80%	0.21	80000
ATTO™ 514	511	532	115.000	85%	0.21	97750
ATTO™520	517	538	110.000	90%	0.16	99000
ATTO™532	532	552	115.000	90%	0.20	103500
ATTO™Rho6G	533	557	115.000	90%	0.19	103500
ATTO™542	542	562	120.000	93%	0.18	111600
ATTO™550	554	576	120.000	80%	0.23	96000
ATTO™565	564	590	120.000	90%	0.27	108000
ATTO™Rho3B	566	589	120.000	50%	0.27	60000
ATTO™Rho11	572	595	120.000	80%	0.26	96000
ATTO™Rho12	577	600	120.000	80%	0.26	96000
ATTO™Thio12	582	607	110.000	15%	0.11	16500
ATTO™Rho101	587	609	120.000	80%	0.18	96000
ATTO™590	593	622	120.000	80%	0.39	96000
ATTO™Rho13	603	626	120.000	85%	0.28	102000
ATTO™594	603	626	120.000	80%	0.22	96000
ATTO™610	616	633	150.000	70%	0.03	105000
ATTO™620	620	642	120.000	50%	0.04	60000
ATTO™Rho14	626	646	140.000	80%	0.26	112000
ATTO™633	630	651	130.000	64%	0.04	83200
ATTO™643	643	665	150.000	62%	0.05	93000
ATTO™647	647	667	120.000	20%	0.08	24000
ATTO™647N	646	664	150.000	65%	0.06	97500
ATTO™655	663	680	125.000	30%	0.24	37500
ATTO™Oxa12	662	681	125.000	30%	0.32	37500
ATTO™665	662	680	160.000	60%	0.07	96000
ATTO™680	681	698	125.000	30%	0.30	37500
ATTO™700	700	716	120.000	25%	0.26	30000
ATTO™725	728	751	120.000	10%	0.08	12000
ATTO™740	743	763	120.000	10%	0.07	12000
CalFluor® Gold540	522	544	0	N/A	N/A	N/A
CalFluor® Orange560	538	559	0	N/A	N/A	N/A
CalFluor® Red590	569	591	0	N/A	N/A	N/A
CalFluor® Red610	590	610	0	N/A	N/A	N/A
CalFluor® Red635	618	637	0	N/A	N/A	N/A
Quasar®670**	647	670	0	N/A	N/A	N/A
Quasar®705**	690	705	0	N/A	N/A	N/A
AlexaFluor®350	346	442	19.000	N/A	0.25	N/A
AlexaFluor®405	401	421	34.000	N/A	0.23	N/A
AlexaFluor®430	434	541	16.000	N/A	N/A	N/A
AlexaFluor®488	495	519	71.000	92%	0.3	65320
AlexaFluor®500	502	525	71.000	N/A	0.32	N/A
AlexaFluor®514	517	542	80.000	N/A	0.31	N/A
AlexaFluor®532	532	554	81.000	61%	0.24	49410
AlexaFluor®546	554	570	112.000	79%	0.21	88480
AlexaFluor®555	555	565	150.000	10%	0.08	15000
AlexaFluor®568	578	603	91.300	69%	0.45	62997
AlexaFluor®594	590	617	90.000	66%	0.43	59400
AlexaFluor®610-X	602	624	132.000	N/A	0.43	N/A
AlexaFluor®633	632	647	100.000	N/A	N/A	N/A
AlexaFluor®647	650	665	239.000	33%	0	78870
AlexaFluor®660	663	690	132.000	37%	0	48840
AlexaFluor®680	679	702	184.000	36%	0	66240
AlexaFluor®700	702	723	192.000	25%	0	48000
AlexaFluor®750	749	775	240.000	12%	0	28800
BODIPY™ /BDP FL	503	509	92.000	97%	0.015	89240
BODIPY™ /BDP TMR	542	574	55.000	64%	0.16	35200
BODIPY™ /BDP 558/568	561	569	84.400	68%	0.00	57392
BODIPY™ /BDP 564/570	565	574	110.000	1%	0.01	1100
BODIPY™ /BDP TR	589	616	69.000	90%	0.15	62100
BODIPY™ /BDP 630/650	628	642	97.000	91%	0.03	88270
BODIPY™ /BDP 650/665	649	667	94.000	52%	0.04	48880

Generated by wpDataTables

* Please note that quantum yield and εmax (and thus brightness) depend on the solvent
** Please note that these are generica of Cy5 and Cy5.5 respectively

ATTO Fluorescent Labels And Dyes

List of Quenchers

Quenchers

Quencher	λ (absorption, max)	λ (absorption, lower to higher end)
DDQI	430	400-530
Dabcyl	453	400-530
BMN-Q460	460	400-530
BHQ®-0	495	430-520
TQ2	520	480-580
EDQ (Eclipse)	522	400-640
MGB™-EQD/Ec	522	400-640
Iowa Black®	530	420-620
BHQ®-1	534	480-580
BMN-Q535	535	480-580
BMN-Q1	535	480-580
TAMRA™*	557	480-580
ATTO 540Q	543	[nm]
DYQ-1	547	[nm]
TQ3	570	550-650
BMN-Q2	580	500-650
BHQ®-2	580	550-650
ATTO 575Q	582	[nm]
ATTO 580Q	587	[nm]
BMN-Q590	590	550-650
ATTO 612Q	615	[nm]
BMN-Q620	620	480-720
DDQII	630	550-720
DYQ-2	641	[nm]
BMN-Q651	650	550-720
BBQ-650	650	550-720
BHQ®-3	670	620-730

Generated by wpDataTables

* TAMRA™ (em, max 580 nm) has some fluorescence in the visible wavelength

DDQ = Deep Dark Quencher
BHQ = Black Hole Quencher
BMN = Biomens
TQ = Tides Quencher
EDQ = Eclipse Dark Quencher

List of Reporter-Quencher Combinations

Quencher

Dye	Abs*	Em*	Dabcyl	BHQ0	Eclipse	MGB-Eclipse	BHQ1	TAMRA	BHQ2	BBQ-650	BHQ3
	[nm]		380-550	430-520	400-640	400-640	480-580	470-560	559-670	550-750	620-730
			453	493	522	522	535	544	579	650	672
FAM™	495	520	X	X	X	X	X	X
TET™	521	536	X	X	X	X	X	X
Yakima Yellow®	530	549	X	X	X	X	X	X
JOE™	529	555	X	X	X	X	X	X		X
HEX™	535	556	X	X	X	X	X	X		X
Cy3®	549	566			X	X			X	X
Cy3B®	560	571			X	X			X	X
TAMRA™	557	583			X	X			X	X
Cy3.5®	581	596			X	X			X	X
ROX™	586	610			X	X		X	X	X
Texas Red®	597	616			X	X			X	X
Cy5®	646	669							X	X	X
Cy5.5®	683	694								X	X
IRDye®700	685	705								X	X
CalFluor®® Gold540	522	544					X
CalFluor® Orange560	538	559					X			X
CalFluor® Red590	569	591							X	X
CalFluor® Red610	590	610							X	X
CalFluor® Red635	618	637							X	X
Quasar®670**	647	670							X	X	X
Quasar®705**	690	705							X	X	X
ATTO™ 390	390	476	X	X
ATTO™ 425	439	476					X
ATTO™ 495	498	526					X
ATTO™ 520	517	538					X
ATTO™ 550	554	576							X	X
ATTO™ Rho12	577	600							X	X
ATTO™ Rho101	587	609							X	X
ATTO™ 594	603	626							X	X
ATTO™ Rho13	603	627							X	X
ATTO™ 610	616	633							X	X
ATTO™ 620	620	642							X	X
ATTO™ 647N	646	664							X	X
Alexa Fluor® 488	490	525					X
Alexa Fluor® 555	555	565							X	X
Alexa Fluor® 647	650	671							X	X	X

Generated by wpDataTables

Internal quenchers and fluorophores are available upon request

* Maximum –
** Please note that these are generics of Cy5 and Cy5.5 respectively

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